Homologous genes, Pe.pleurotolysin A and Pe.ostreolysin, are both specifically and highly expressed in primordia and young fruiting bodies of Pleurotus eryngii

•We identified two genes of aegerolysin-family protein in Pleurotus eryngii.•Pe.PlyA and Pe.ostreolysin were both highly expressed in primordia of P. eryngii.•Pe.PlyA and Pe.ostreolysin from P. eryngii are orthologues of P. ostreatus.•Pe.PlyA and Pe.ostreolysin in P. eryngii are paralogous genes.

We identified two aegerolysins in the transcriptome of Pleurotus eryngii that are homologs of pleurotolysin A (PlyA) and ostreolysin in P. ostreatus. The amino acid sequence of Pe.PlyA was highly homologous to that of PlyA, and Pe.ostreolysin was highly homologous to that of ostreolysin. Furthermore, these genes were both specifically and highly expressed in primordia of P. eryngii in the same manner as in P. ostreatus. The analyses revealed that Pe.PlyA and Pe.ostreolysin from P. eryngii are functional orthologues of PlyA and ostreolysin from P. ostreatus. These analyses also revealed that paralogous relationship of Pe.PlyA and Pe.ostreolysin.