Identification of a mycorrhizal fungus in Epipogium roseum (Orchidaceae) from morphological characteristics of basidiomata

A mycorrhizal fungus, ME1-1, isolated from an achlorophyllous orchid, Epipogium roseum, was identified as Coprinellus disseminatus ( Coprinus disseminatus) based on characteristics of basidiomata that were artificially induced. Spawn of ME1-1 cultivated on a medium that consisted of sawdust and wheat, sandwiched between two mats of volcanic soils, which was incubated at 20.0° ± 0.5°C in 80.0% ± 0.5% relative humidity in the dark. The basidiomata were formed on the soil after 2 months. Morphological and anatomical characteristics of the basidioma mostly accorded with those of Coprinellus disseminatus. We therefore concluded that C. disseminatus is one of the mycobionts of E. roseum.