Article ID Journal Published Year Pages File Type
2062879 Reproductive Biology 2009 10 Pages PDF
Abstract

SUMMARYThe aims of the study were 1/to isolate and identify equine seminal plasma proteinase inhibitors, 2/to evaluate their inhibitory potential, and 3/to test a correlation between protein concentration in seminal plasma supernatant (obtained after precipitation with 36% ammonium sulfate) and stallion sexual maturity. Seminal plasma proteins obtained from six stallions were chromatographed in a Superose 12 (FPLC system) column followed by C18 HPLC reverse-phase. Inhibition of trypsin amidase activity was evaluated in the collected fractions. Active proteins with a molecular mass of 6.3-7.0 kDa were identified using mass spectrometry. The older stallions showed a reduction in total seminal plasma protein concentration, but had similar concentrations of proteinase inhibitors (0.28+0.10 mg/ml) in seminal plasma supernatant. Different proteinase inhibitor isoforms were found in semen of all stallions which suggests that the isoforms may be used as biomarkers of individual animals.

Related Topics
Life Sciences Agricultural and Biological Sciences Animal Science and Zoology
Authors
, , , , ,