Saccharopolyspora endophytica sp. nov., an endophytic actinomycete isolated from the root of Maytenus austroyunnanensis

A Gram-positive, aerobic, non-motile, non-acid–alcohol-fast strain, designated YIM 61095T, was isolated from the root of Maytenus austroyunnanensis collected from a tropical rainforest of Xishuangbanna in Yunnan Province, south-west China. Strain YIM 61095T exhibited chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61095T was a member of the genus Saccharopolyspora and was most closely related to Saccharopolyspora flava AS 4.1520T (97.7% sequence similarity). The major fatty acids were iso-C15:0, iso-C16:0, iso-C17:0 and anteiso-C17:0. The predominant quinone detected was MK-9(H4). The DNA G+C content was 66.2 mol%. The phenotypic characteristics and DNA–DNA hybridization relatedness data indicated that strain YIM 61095T should be distinguished from Saccharopolyspora flava AS 4.1520T. On the basis of the evidence presented in this study, strain YIM 61095T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora endophytica sp. nov. is proposed. The type strain is YIM 61095T (=KCTC 19397T=CCTCC AA 208003T).