| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2072455 | Animal Reproduction Science | 2016 | 7 Pages |
The climbing perch, Anabas testudineus, is an air-breathing fish having great consumer preference as a food fish and is considered a prime candidate species for aquaculture. Spawning success is an important issue while using hormones for captive induced breeding. In the first experiment, a trial was conducted to assess the efficacy of a synthetic Gonadotropin Releasing Hormone analog (sGnRHa) on the spawning success of climbing perch. Female fish were administered six different doses each with a single intramuscular injection of sGnRHa hormone at 0.002 (TOD1), 0.005 (TOD2), 0.01 (TOD3), 0.015 (TOD4), 0.02 (TOD5), 0.03 (TOD6) μg/g body weight. Similarly, males were administered half of the hormone dose of females in all the respective treatment groups. The greatest (P < 0.05) relative fecundity (715.13 ± 15.0 eggs/g female body weight) and fertilization percentage rates (93.1 ± 8.0%) occurred when female fish were treated at the 0.015 μg/g body weight dose. There was a reduction in relative fecundity and hatching rate in female fish injected with the largest dose (1.5 μL/g body weight) of sGnRHa. A second experiment was conducted to assess the effect of a different male-female ratio on optimum spawning success in climbing perch. For this study a different female to male ratio (1:1, 1:2, 1:3 and 1:4) and male to female ratio (1:1, 1:2 and 1:3) were used. There were a greater (P < 0.05) relative fecundity (886.62 ± 17.9 eggs/g female body weight), fertilization (98 ± 6.7%) and hatching (99 ± 5.4%) rates with the female to male ratio of 1:2. This indicated that the hormone dose of 0.015 μg/g body weight and a female-male ratio of 1:2 are optimal for enhanced spawning success in the climbing perch.
