Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2074215 | Animal Reproduction Science | 2009 | 10 Pages |
Abstract
The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E™). Sperm motility, sperm chromatin structure, membrane integrity and mitochondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E™ significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI.
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Authors
B. Macías García, J.M. Morrell, C. Ortega-Ferrusola, L. González-Fernández, J.A. Tapia, H. Rodriguez-Martínez, F.J. Peña,