Article ID Journal Published Year Pages File Type
2074629 Animal Reproduction Science 2007 9 Pages PDF
Abstract

An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse®, PG600® and Chorulon® along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10–16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 one-cell embryos recovered per animal.

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Life Sciences Agricultural and Biological Sciences Animal Science and Zoology
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