| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2075205 | Animal Reproduction Science | 2007 | 14 Pages |
The aim of this study was to determine the prostaglandins (PGs) production and ovarian function in gilts after intrauterine infusions of 106 and 109 colony-forming units (cfu)/ml of Escherichia coli (E. coli). In Experiments 1 and 2, 30 ml of saline or 30 ml of E. coli suspension containing 106 or 109 cfu/ml, were infused once into each uterine horn in three groups of gilts on day 3 of the estrous cycle, respectively. In Experiment 1, 17 days after treatment it was revealed that inoculation of E. coli 109 cfu/ml induced severe acute or subacute endometritis while 106 cfu of E. coli evoked moderate acute endometritis or resulted in no inflammatory changes. In the gilts receiving 109 cfu/ml of E. coli, the concentration of 13,14-dihydro-15-keto-PGF2α in blood from the jugular vein was elevated (P < 0.05–0.001) compared to concentration in the gilts inoculated with 106 cfu on days 8–17 after treatment. Both the E. coli-treated groups had a lower (P < 0.05, P < 0.01) progesterone plasma level from days 10 to 14 after administration than the control group. On day 17 of the study, infusion of E. coli 109 cfu/ml, in comparison to 106 cfu, resulted in the greater (P < 0.001) content of PGE2 in the myometrium. The content of both PGs in the endometrium as well as PGF2α in the myometrium of gilts-treated with 109 cfu/ml of E. coli was lower (P < 0.001) than in gilts-treated with 106 cfu of bacteria. Newly formed corpora lutea were found in the gilts infused with 106, but not those infused with 109 cfu/ml of E. coli on day 17 after infusion. On day 8 of the study (Experiment 2), the blood from utero-ovarian vein of the gilts-treated with 109 cfu/ml of bacteria had a higher (P < 0.05) PGF2α level and lower (P < 0.001) PGE2 level than following infusion of E. coli 106 cfu/ml. Also on day 8 of the study, the content of PGE2 in the endometrium, both the PGs in the myometrium as well as cyclooxygenase-2 in the endometrium and myometrium was greater (P < 0.01, P < 0.001) after applying 109 cfu/ml than 106 cfu/ml of E. coli. These results indicate that intrauterine infusions of 106 or 109 cfu/ml of E. coli lead to the development of inflammatory states of different intensities which is connected with different PGF2α and PGE2 production and function of ovaries.
