| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2075425 | Biocatalysis and Agricultural Biotechnology | 2014 | 4 Pages |
•Immobilization of glucose isomerase on smooth and rough glass beads.•Immobilization yield is significant.•Comparison of immobilization of glucose isomerase on smooth and rough glass beads.
Immobilization of glucose isomerase (GI) (EC 5.3.1.5) has been carried out on non-porous glass surfaces using γ-aminopropyltriethoxysilane and polyethyleneimine as an activator and gluteraldehyde as cross linking agent for its potential use. Polyethyleneimine was found to be a superior immobilization activator than γ-aminopropyltriethoxysilane. Further, the effects of rough and smooth surfaces of non-porous glass beads on immobilization of glucose isomerase have been investigated. Polyethyleneimine on rough glass beads was found to be better activator material than that of γ-aminopropyltriethoxysilane. The prime objective of study was to immobilize glucose isomerase on an improved surface of non-porous glass beads which will give a significantly higher immobilization yield. After optimizing the parameters the immobilization yield increased from an initial value 29.43% to 79.6%.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
