Studies on endosulfan degradation by local isolate Pseudomonas aeruginosa

A bacterium capable of tolerating high levels of endosulfan was isolated from soil of cotton field. The ability of the bacterium to degrade endosulfan was investigated by culturing it in minimal media and nutrient broth containing high levels of endosulfan. The 16S rRNA gene sequencing indicated the bacterium as Pseudomonas aeruginosa. Endosulfan degradation by the bacteria was found to occur more rapidly in media with supportive nutrients than the minimal media. P. aeruginosa was able to degrade 96% of endosulfan after 288 h under static conditions. The metabolites formed during endosulfan degradation were analyzed by using UV visible spectrophotometry and thin layer chromatography. The complete mineralization of endosulfan was found to occur after 28 days. The levels of dehydrogenase, arylsulfatase, dehalogenase enzymes and CO2 were monitored to test the soil activity. The terminal products formed by bacteria during biodegradation were tested for phyto-toxicity. This study will be useful for the development of bioremediation strategies.