Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2075518 | Biocatalysis and Agricultural Biotechnology | 2012 | 5 Pages |
Abstract
Batch cultivation of the fungus Piriformospora indica on Kaefer medium containing soluble starch resulted in a 1.5-fold improvement in amylase activity and a reduction in the time required for achieving maximum growth, amylase activity and spore yield by more than 50%. Peak growth (7.64 g/L) and amylase activity (39.84 U/mL) were recorded after 42 h while maximum spore yield (8.78×107 spores/mL) was achieved after 60 h of cultivation. The optimum temperature and pH for amylase activity was 80 °C and 7.0, respectively, with about, 80% of activity retained at 90 °C and 70% of activity retained at pH 9.0.
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Authors
Vinod Kumar, Vikram Sahai, V.S. Bisaria,