Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2075537 | Biocatalysis and Agricultural Biotechnology | 2012 | 6 Pages |
Eugenol caprylate was synthesized using Lipozyme TLIM as the biocatalyst for the reaction. A two level Plackett–Burman (PB) experimental design was used for finding the significant reaction parameters. Response surface methodology (RSM) with a three-factor-five-level central composite rotatable design (CCRD) was further employed to study and optimize the reaction conditions. A good correlation between the predicted and actual responses showed that the generated model could adequately predict the conversion yield. The maximum conversion yield (72.2%) was obtained at the optimal condition of 65 °C, 250 rpm, 259 min, 100 mg enzyme, and 2:1 M ratio of eugenol/caprylic acid in solvent-free system.
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