| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2075558 | Biocatalysis and Agricultural Biotechnology | 2013 | 7 Pages |
Our study focused towards screening the modes of nutrition of Chlorella sorokiniana under standard laboratory conditions and its upscaling in a photobioreactor for enhanced lipid productivity. Time course studies using glucose under mixotrophic and heterotrophic conditions vis a vis autotrophy revealed highest values of growth, lipid productivity (29.33% DCW) and lipid content (755. 00 mg/L) under mixotrophic conditions on the 14th day of growth. Upscaling in a photobioreactor with selected substrates (Glucose and tryptophan), along with sodium thiosulphate as a reducing agent was undertaken. Lipid productivity of 34.16% and 36.49% was recorded after 4th and 8th day, respectively, in both substrates, vis a vis 11% with glucose alone. FAME analyses revealed a 2% enhancement in total saturated fatty acids (SFA) in tryptophan supplemented culture, which also exhibited an almost ideal fatty acid ratio for use as biodiesel (2.6:4:1 of 16:1.18:1 and 14:1). Interestingly, glucose+sodium thiosulphate grown culture exhibited an almost 2 folds enhancement in PUFA content (especially 18:1 and 18:2), illustrating its promise for use as an edible oil blend. Our study revealed the step-wise scale up and promise of substrate amendment and mixotrophy as promising tools for valorization of microalgal lipids.
