Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2075623 | Biocatalysis and Agricultural Biotechnology | 2016 | 9 Pages |
Abstract
The enzyme precipitated successfully with both polymers. The precipitation method applied to commercial endoglucanase and the fungal extract showed similar patterns with high purification factors. The recovery of the activity in the re-dissolved precipitate from the fungal extract was close to 40% at pH 5.3 using PVS (1% w/w) as precipitating agent and the purification factor was near 9. The purification factor of endoglucanase in the precipitate of the enzymatic extract from SSF with CHS (0.05% w/v) was around 7. These parameters make this precipitation method appropriate to be included in the last stages of a downstream process, with advantages such as simplicity, scalability and ability to concentrate and stabilize the enzyme.
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Agricultural and Biological Sciences (General)
Authors
MarÃa Julia Boggione, Ramiro Becher, Beatriz Farruggia,