Article ID Journal Published Year Pages File Type
2075647 Biocatalysis and Agricultural Biotechnology 2015 6 Pages PDF
Abstract

Centrifugal ultrafiltration of genipap fruit extracts after mechanical/sonic enzymatic treatment was performed through 50, 30, 10 and 3 kDa molecular weight cutoff (MWCO) (15 min; 10 °C; 9425g). Volume, volume retention factor (VRF), flux, genipin and glucose concentrations were determined. Separation efficiency, retention, transmission coefficients (RC, TC), and membrane selectivity were estimated. Reduction in MWCO increased volume in the retentate (7.6±0.0 mL), decreased VRF (1.9±0.0) and flux (0.06±0.00 mL cm−2 min−1) after 3 kDa ultrafiltration. Genipin and glucose reached equilibrium in retentate and permeate (17±3.3; 16±1.5 mg mL−1; 41±2.2 and 41±4.8 mg mL−1) after 3 kDa ultrafiltration. The highest removal of genipin was 0.5±0.1. RC and TC were 0.5±0.1; selectivity for genipin was 0.9±0.0 after 3 kDa separation.

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