Enhanced production of β-d-fructofuranosidase by Saccharomyces cerevisiae using agro-industrial wastes as substrates

Agro-industrial wastes as carbon and nitrogen sources are evaluated to develop a cost effective media formulation for β-d-fructofuranosidase (FFase) production by Saccharomyces cerevisiae GVT263 to replace more costly sucrose, peptone, yeast extract and malt extract. Banana leaf powder (BL) and groundnut oil cake (GOC), which were significant and promising carbon and nitrogen sources for FFase production along with MnSO4, inoculum size (IS) and incubation period (IP) are selected and optimized. Maximum FFase production with 9-fold increase (from 400 U mL−1 in basal medium to 3587 U mL−1) was obtained using BL 4%, GOC 4%, MnSO4 0.06%, IS 0.5% in 48 h. This is the first report on FFase production using BL and GOC as sole source of carbon and nitrogen.