Article ID Journal Published Year Pages File Type
2075690 Biocatalysis and Agricultural Biotechnology 2012 7 Pages PDF
Abstract

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25 h, 66 °C, enzyme dosage 3.1%w/w.

Related Topics
Life Sciences Agricultural and Biological Sciences Agricultural and Biological Sciences (General)
Authors
, , ,