Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2093922 | Stem Cell Research | 2015 | 4 Pages |
Abstract
Here we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies.
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Authors
Rosa Montes, Tamara Romero, Sonia Cabrera, Veronica Ayllon, Jose A. Lopez-Escamez, Veronica Ramos-Mejia, Pedro J. Real,