Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2093924 | Stem Cell Research | 2015 | 7 Pages |
Abstract
We developed buffalo embryonic stem cell lines from somatic cell nuclear transfer derived blastocysts, produced by hand-guided cloning technique. The inner cell mass of the blastocyst was cut mechanically using a Microblade and cultured onto feeder cells in buffalo embryonic stem (ES) cell culture medium at 38 °C in a 5% CO2 incubator. The stem cell colonies were characterized for alkaline phosphatase activity, karyotype, pluripotency and self-renewal markers like OCT4, NANOG, SOX2, c-Myc, FOXD3, SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 and CD90. The cell lines also possessed the capability to differentiate across all the three germ layers under spontaneous differentiation conditions.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Syed Mohmad Shah, Neha Saini, Syma Ashraf, Manoj K Singh, Radheysham Manik, Suresh K Singla, Prabhat Palta, Manmohan Singh Chauhan,