| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2093970 | Stem Cell Research | 2016 | 4 Pages |
Abstract
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
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Authors
María Questa, Leonardo Romorini, Carolina Blüguermann, Claudia María Solari, Gabriel Neiman, Carlos Luzzani, María Élida Scassa, Gustavo Emilio Sevlever, Alejandra Sonia Guberman, Santiago Gabriel Miriuka,