| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2093975 | Stem Cell Research | 2016 | 4 Pages |
Abstract
The Genea042 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype and female allele pattern through traditional karyotyping, CGH and STR analysis. Pluripotency of Genea042 was demonstrated with 81% of cells expressing Nanog, 95% Oct4, 53% Tra1-60 and 97% SSEA4, a PluriTest Pluripotency score of 30.06, Novelty score of 1.24 and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.
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Authors
Biljana Dumevska, Omar Chami, Robert McKernan, Divya Goel, Uli Schmidt,