Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2093984 | Stem Cell Research | 2016 | 4 Pages |
Abstract
The Genea021 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Trisomy 21, indicative of Down Syndrome. Following ICM outgrowth on inactivated human feeders, CGH and STR analyses demonstrated a 47, XY, + 21 karyotype and male allele pattern. The hESC line had pluripotent cell morphology, 71% of cells expressed Nanog, 84% Oct4, 23% Tra1–60 and 95% SSEA4, gave a Pluritest Pluripotency score of 21.85, Novelty of 1.42, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
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Authors
Biljana Dumevska, Alexis Bosman, Robert McKernan, Heather Main, Uli Schmidt, Teija Peura,