| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2093998 | Stem Cell Research | 2016 | 4 Pages |
Abstract
The Genea022 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea022 was demonstrated with 84% of cells expressed Nanog, 98% Oct4, 55% Tra1–60 and 97% SSEA4, gave a Pluritest Pluripotency score of 42.95, Novelty of 1.23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
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Authors
Biljana Dumevska, Alexis Bosman, Robert McKernan, Uli Schmidt, Teija Peura,