| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2094003 | Stem Cell Research | 2016 | 4 Pages |
Abstract
The Genea017 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 40 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, genetic analysis confirmed a 46, XY karyotype and male allele pattern through CGH and STR analysis. The hESC line had pluripotent cell morphology, 87% of cells expressed Nanog, 95% Oct4, 88% Tra1-60 and 99% SSEA4, gave a PluriTest pluripotency score of 34.74, novelty of 1.27, demonstrated alkaline phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
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Authors
Biljana Dumevska, Robert McKernan, Divya Goel, Uli Schmidt, Teija Peura,