| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2094006 | Stem Cell Research | 2016 | 4 Pages |
Abstract
The Genea050 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying a deletion in 4q35 with only 5 D4Z4 repeats by PGD linkage analysis, indicative of FSHD1. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XY and STR analysis demonstrated a male Allele pattern. The hESC line had pluripotent cell morphology, 92% of cells expressed Nanog, 97% Oct4, 79% Tra1-60 and 99% SSEA4, gave a Pluritest Pluripotency score of 25.45, Novelty of 1.45 demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
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Authors
Biljana Dumevska, Omar Chami, Heather Main, Robert McKernan, Divya Goel, Uli Schmidt,