Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2094115 | Stem Cell Research | 2016 | 4 Pages |
Abstract
Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, RDEB-iPSC26, was confirmed by the expressions of stem cell markers and the differentiation capability into three germ layer. RDEB-iPSC26 may be a useful cell resource for the establishment of in vitro RDEB modeling and the study for developing gene and cell therapy.
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Authors
Munenari Itoh, Shiho Kawagoe, Katsuto Tamai, Hirotaka James Okano, Hidemi Nakagawa,