Article ID Journal Published Year Pages File Type
2094124 Stem Cell Research 2016 4 Pages PDF
Abstract

We have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1+ cell populations.

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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing
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