| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2094321 | Stem Cell Research | 2016 | 5 Pages |
Abstract
Human bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors — TGFβ receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3β inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Pluritest.
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Authors
Matthias Megges, Richard O.C. Oreffo, James Adjaye,