Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2095167 | Theriogenology | 2015 | 8 Pages |
Abstract
The common marmoset, Callithrix jacchus, is used as a New World monkey species in biomedical studies because of its small body size and good reproduction in captivity. A modified artificial insemination technique was developed in this species to encourage breeding of lines carrying interesting genes and traits. Fresh semen was collected by penile vibratory stimulation. Medium containing highly motile sperm was inseminated into the uterus using a catheter. Seven females were inseminated using freshly prepared sperm from different males every day for 3Â days including the expected ovulation day. As a result, four females conceived, and three females delivered six offspring in total (two singletons and one quadruplet). The paternity of the newborns was determined using microsatellite markers to accurately pinpoint the timing of insemination and ovulation. It is expected that our artificial insemination protocol can be effectively used to establish marmoset lines and genetically manage marmoset colonies.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
Shuji Takabayashi, Yuiko Suzuki, Hideki Katoh,