| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2095627 | Theriogenology | 2009 | 12 Pages |
The objective of this study was to investigate the effects of polyvinylpyrrolidone (PVP) on sperm capacitation status and embryonic development in cattle (Bos taurus). Acrosome-reacted sperm (chlortetracycline stain) and the fertilization rate after intracytoplasmic sperm injection (ICSI) were enhanced (P < 0.05) when sperm were cultured in a medium containing 10% PVP. However, injection of bovine in vitro–produced (IVP) embryos with medium containing 10% PVP suppressed development of these embryos to the 2-cell, morula, and blastocyst stages and cell number at the blastocyst stage (P < 0.01) but did not affect chromosomal integrity (P > 0.05). Embryo developmental capacity differed (P < 0.01) among PVP from three suppliers, but there were no significant differences among three solvent media. The PVP remained localized in 40.9% of PVP-injected IVP embryos. In conclusion, PVP affected the acrosome reaction and enhanced the fertilization rate after ICSI. However, PVP remained detectable in IVP embryos and suppressed their developmental capacity.
