| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2097253 | Theriogenology | 2006 | 9 Pages |
Two trials were conducted to ascertain fertilization rate, embryo quality and numbers of transferable embryos in superovulated heifers and cows inseminated with sexed sperm. Inseminates contained 2 × 106, 10 × 106 or 20 × 106 total sperm enriched for the X- or Y-chromosome (∼90%) by flow cytometry/cell sorting. Non-sexed inseminates contained 40 × 106 total sperm. Donors in each trial were allocated to one of each of the bulls included in that study. Each donor was inseminated with frozen/thawed sperm from the same bull for each treatment in successive courses of superstimulation with twice daily i.m. injections of FSH for 4 d. Heifers and cows were inseminated 12 and 24 h after visually observed standing estrus in Trial 1. In Trial 2, a single timed inseminate was used 70–72 h following PGF2α. Ova/embryos were collected non-surgically 7–7.5 d after insemination. In both trials, fewer ova were fertilized with sexed versus non-sexed treatments and with 2 × 106 sexed sperm compared to higher doses (P < 0.05). However, insemination of 20 × 106 total sexed sperm of ≥90% purity resulted in similar numbers of transferable embryos of the desired sex compared to that for non-sexed sperm.
