Article ID Journal Published Year Pages File Type
2097514 Theriogenology 2005 10 Pages PDF
Abstract

The objective of this study was to assess the protein profile of ovine seminal plasma using 2D-PAGE and verify if BSP A1/A2 are present in ovine seminal plasma. Seminal plasma was collected from three mature rams and pooled to eliminate individual differences. Seminal plasma samples were submited to 2D-PAGE using 12% acrylamide gels. The image analysis software identified 21 protein spots on the air-dried gel, with molecular weight ranging from 15 to 115 kDa and pI 3.2 to 8.7. The most prominent spots were those <30 kDa. The most intensely stained spots were: 3 (18–19 kDa, pI 4.8–5.0), 5 (17–18 kDa, pI 5.0–5.2), 7 (15–16 kDa, pI 6.2–6.4), and 23 (105–108 kDa, pI 6.8–7.0). Three of these spots (spots 3, 5 and 7, respectively) accounted for 41.1% of the relative intensity of the spots of the gels, based on the intensity of the Comassie blue staining. Western blot analysis indicated that spots 3 and 5 were similar to BSP A1/A2 (16.5, pI 4.7–5.0 and 16 kDa, pI 4.9–5.2) identified in Manjunath's studies [Manjunath P, Sairam MR. Purification and biochimical characterization of three major acid proteins (BSP A1, BSP A2 and BSP A3) from bovine seminal plasma. Biochem J 7 (1987) 685–92.], based on the specific reaction of the polyclonal antibody to those spots.

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