| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2203569 | Tissue and Cell | 2015 | 6 Pages |
•We investigate the presence of Osx, HNK1 and Sox10 in odontogenesis and osteogenesis.•Osx and HNK1 are present in undifferentiated cells in osteogenesis and odontogenesis.•Sox10 is present in undifferentiated cells only in the early stages analyzed.•Osx, HNK1 and Sox10 are present in osteoblasts, odontoblasts and ameloblasts.•They are possible related to cell differentiation during the matrix secretion process.
Cell differentiation is essential for maxillaries and tooth development. Facial mesenchymal tissue is formed by neural crest cells (NC). These cells are highly migratory, giving rise to various cell types, considered with a high level of plasticity, indicating that they contain progenitor cells with a great power of differentiation. In this study, it was analyzed the presence of NC cell progenitors and mesenchymal stem cells (MSC) during maxillaries osteogenesis and odontogenesis in rats. Histological slides were collected in two phases: embryonic age of 15 and 17 days; 2, 4 and 7 days after birth. Immunohistochemistry for MSC markers (Osterix) and NC cells (Sox10, HNK1) was performed. The results showed positive expression for Osterix and HNK1 in undifferentiated ectomesenchymal cells in early and late stages; Sox10 was present only in early stages in undifferentiated cells. All markers were present in differentiated cells. Although the experiments performed do not allow us to explain a possible role for Osx, HNK1 and Sox10 in both differentiated and undifferentiated cells during osteogenesis and odontogenesis, it had shown important results not yet described: the presence of HNK1 and Sox10 in osteoblasts and odontoblasts in late development stages and in the tooth germ epithelial cells and ameloblasts.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (116 K)Download as PowerPoint slide
