| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2203639 | Tissue and Cell | 2014 | 5 Pages |
•We examine distribution of YB-1 in the nuclei of different cells.•Nuclear distribution pattern of YB-1 differs in different cell types.•YB-1 may be a component of Cajal bodies in embryos, but not in other cells studied.•YB-1 is partially colocalized with RNA polymerase I in some cell types.
In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied.
