Article ID Journal Published Year Pages File Type
2203687 Tissue and Cell 2015 7 Pages PDF
Abstract
In this research, mouse BMMSCs were isolated from bone marrow, induced to differentiate into neurogenic cells in vitro, and transplanted into the injured spinal cord after over-expression of miR-124. The results showed that the BMMSCs could induce the differentiation to neurogenic cells under the special condition medium, but when the miR-124 was over-expressed, the differentiation efficiency of neurogenic cells from BMMSCs could be promoted. This reason was demonstrated that polypyrimidine tract-binding protein 1 (PTBP1) showed a repressor for polypyrimidine tract-binding protein 2 (PTBP2) during neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. miR-124 promoted neurogenic cells from BMMSCs were successful colonized into injured spinal cord for participation in tissue-repair. In conclusion, our research shows that the miR-124 promoted the differentiation of neuronal cells from BMMSCs, and this mechanism was miR-124 reduced the expression of PTBP1, increased the expression of PTBP2.
Related Topics
Life Sciences Agricultural and Biological Sciences Agricultural and Biological Sciences (General)
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