| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2203824 | Tissue and Cell | 2014 | 6 Pages |
•The effective recipient model for SSCs transplantation in Kunming mice was established after different doses busulfan treatment.•The optimal dose of busulfan was 20 mg/kg and the most appropriate time for transplantation was 5–7 wk after busulfan treatment.•Donor-derived cloned fragments in the testis of recipient mice and offspring were produced.
The objective of this study was to establish a recipient model for spermatogonial stem cells (SSCs) transplantation in the Kunming mice after different doses busulfan treatment. The results showed that the most optimal dose of busulfan was 20 mg/kg and the most appropriate time for transplantation was 5–7 wk after busulfan treatment. Then, the cloned fragments existed in the testis of recipient mice after 20 mg/kg busulfan treatment and the offspring with enhanced green fluorescent protein (EGFP) were produced by the transplanting SSCs. Hence, we established the effective recipient model for donor-derived SSCs transplantation in Kunming mice.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (303 K)Download as PowerPoint slide
