| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2394082 | Domestic Animal Endocrinology | 2006 | 10 Pages |
Abstract
Feline proinsulin was cloned and expressed using a bacterial expression system. It was then purified from inclusion bodies using size exclusion chromatography and further processed including reduction of the protein. Following refolding, proinsulin was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). RP-HPLC and mass spectrometric analysis indicated that the proinsulin contained the correct disulfide bridging pattern. This proinsulin can be used for therapeutic and diagnostic purposes.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
M. Hoenig, Z.F. Caffall, R.A. McGraw, D.C. Ferguson,