Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2394322 | Journal of Equine Veterinary Science | 2016 | 4 Pages |
Abstract
The purpose of this study was to provide data on the disposition of stanozolol after bilateral intra-articular injection in the tarsal joints, to discover the length of time for which the drug can be detected in plasma after administration. Fourteen horses were included in the study. After aseptically preparing the injection site, 1Â mL of an aqueous suspension containing 5Â mg of stanozolol was injected into both the right and left tarsal joints of 12 horses; the two remaining animals were not treated and were used as a control group. Five milliliters of blood was collected immediately before stanozolol administration (t0) and at 1, 2, 4, 6, 12, 18, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 144, 156, and 168Â hours after injection. The plasma concentration was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after solid phase extraction. The maximum plasma concentration was 1.7Â ng/mL (range, 0.5-3.0Â ng/mL), measured at 6Â hours (range, 4-12Â hours). The plasma elimination half life varied between 4 and 12Â hours, whereas the plasma clearance per fraction of dose absorbed was in the 257.85-820.88Â L/h range. The results of the present study make a preliminary contribution toward understanding the elimination profile of intra-articularly administered stanozolol in the horse. The drug passes rapidly into the systemic circulation, is eliminated rapidly, and is detected in plasma for no more than 36Â hours after local administration.
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Authors
Noemi Romagnoli, Anna Zaghini, Giorgio Fedrizzi, Adriano Sala, Simona Babbini, Andrea Barbarossa,