Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2398515 | Journal of Feline Medicine & Surgery | 2007 | 6 Pages |
Abstract
In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.
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Authors
Mark D.G. Pinches, Christopher R. Helps, Tim J. Gruffydd-Jones, Kathy Egan, Oswald Jarrett, Séverine Tasker,