Changes in soil carbon of pastures after afforestation with mixed species: Sampling, heterogeneity and surrogates

Accurate and efficient estimation of soil C is vital to understanding and monitoring the role of afforestation in C sequestration. Here, we focused on the potential of mixed-species plantings, for which there is negligible information but expanding investment due to their added environmental benefits. We surveyed soil C and N over a representative chronosequence (5–29 years old) of existing plantings, including measurements in the adjacent pastures to account for differences in soil type and land-use history among properties. Vegetation characteristics of the tree plantings were measured to identify potential surrogates for rapid assessment of soil C. Soil C was highly heterogeneous under the plantings and the adjacent pastures, with up to eight cores required to sample adequately a plot of 400 m2. Vegetation surrogates had limited success in predicting soil C after afforestation, with the only strong predictors being tree density and planting age. Three decades of afforestation with mixed species had not led to substantial changes in C concentration or content of the soil. The C:N ratio of soils increased with planting age suggesting that the C becomes more resistant to decomposition after afforestation. Over longer time scales, tree plantings are likely to have larger impacts on the amount and forms of soil C.

► We surveyed soil carbon in a chronosequence (5–29 years old) of mixed-species plantings. ► Vegetation characteristics were measured to identify surrogates for soil carbon. ► Soil carbon was heterogeneous but was sampled adequately with less than ten cores. ► Tree density and planting age were strong predictors of soil carbon. ► C:N ratio but not C concentration or content of soils increased with planting age.