| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2419485 | Animal Feed Science and Technology | 2015 | 9 Pages |
•A xylanase from rumen microbiome was successfully surface displayed on yeast.•Yeast surface displayed xylanase had great xylanolytic ability producing xylose and xylobiose.•Yeast surface displayed xylanase improved corn stover digestion and fermentation.
This study aimed to develop a yeast strain that surface displays a xylanase that was found from a rumen fosmid library and optimized by directed evolution (Orf6-unm). The Orf6-unm enzyme was successfully surface-displayed using Saccharomyces cerevisiae EBY100 as host (referred to as EBY100-pYD1-orf6-unm), yielding a specific xylanase activity of 137 U/g dry cells. The EBY100-pYD1-orf6-unm had greater xylanolytic activity and produced more xylose from beechwood xylan than the purified Orf6-unm overexpressed in Escherichia coli. The EBY100-pYD1-orf6-unm was evaluated for its effect on digestion of corn stover by in vitro rumen cultures. Both EBY100 and EBY100-pYD1-orf6-unm increased volatile fatty acid production, dry matter degradation, and total bacteria population, while shortening the lag time of gas production. However, EBY100-pYD1-orf6-unm increased both gas production and dry matter degradation, and shortened the lag time to greater magnitudes than EBY100. EBY100-pYD1-orf6-unm may be used to deliver both xylanase and live yeast to feed animals.
