| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2422362 | Aquaculture | 2012 | 4 Pages |
Nervous necrosis virus (NNV), the causative agent of viral nervous necrosis (VNN), is an indigenous virus in Korean coastal areas and a threat to the farming industry. In the present study, we investigated the curative and preventive effects against VNN in sevenband grouper (Epinephelus septemfasciatus) using polyinosinic polycytidylic acid [Poly(I:C)]. Fish challenged with brain-filtrates from VNN-affected fish at 0, 2, and 4 d after Poly(I:C) injection showed < 10% cumulative mortality, whereas fish that were not administered Poly(I:C) but challenged 14 d after the Poly(I:C) injection showed 100% and 80% cumulative mortality, respectively. The surviving fish from the primary challenge with brain-filtrates following Poly(I:C) injection were highly protected from re-challenge at 15 and 30 d post-primary challenge. Moreover, fish pre-immersed with red-spotted grouper nervous necrosis virus (RGNNV) survived following Poly(I:C) administration, even though their clinical signs did not recover. From these results, it was confirmed that sevenband grouper were highly protected from RGNNV challenge by Poly(I:C) administration.
► We researched curative and preventive effects of Poly(I:C) for VNN in grouper. ► Fish injected with Poly(I:C) were highly protected from NNV challenge. ► Surviving fish, NNV challenged following Poly(I:C) injection protected NNV. ► Fish pre-immersed with NNV survived due to Poly (I:C) administration.
