| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2423039 | Aquaculture | 2011 | 6 Pages |
Juvenile silver fish (Rhamdia quelen) were exposed to the essential oil of Lippia alba and transported in plastic bags (10 specimens per bag, load density of 140–200 g.L−1) for different periods (5, 6 and 7 h) yielding final different oxygen levels. The biomarkers of oxidative stress, lipoperoxidation (LPO), catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST) were measured in the liver, gills and brain of the fish. The juveniles were assigned to 6 different treatment groups according to the presence or not of the essential oil of L. alba in water (10 μL L−1) and the length of transportation, which determined the final concentration of dissolved oxygen inside the bags: Five hours: hyperoxia (13.25 ± 0.35 mg L−1 O2); hyperoxia with L. alba (11.27 ± 0.22 mg L−1 O2); Six hours: normoxia (7.35 ± 0.35 mg L−1 O2); normoxia with L. alba (7.29 ± 0.40 mg L−1 O2); Seven hours: hypoxia (2.29 ± 0.36 mg L−1 O2); hypoxia with L. alba (3.82 ± 0.7 mg L−1 O2). The presence of essential oil of L. alba causes an increase of LPO in the brain of fish transported for 5 h and a decrease of GST in the fish transported for 5 and 7 h as compared to those transported for 6 h. In the liver, this essential oil of L. alba decreased LPO as well the three antioxidants enzymes measured in fish transported for 5 h whilst in the gills there was a decrease of LPO in fish transported for 5 and 7 h. These results suggest that the presence of the essential oil of L. alba improves the redox state in the evaluated tissues, both under hyperoxia and under hypoxia.
