| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2423256 | Aquaculture | 2011 | 9 Pages |
The formation of DNA adducts 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) and 1,N6-etheno-2′-deoxyadenosine (εdAdo) in organs of rainbow trout fed with different lipid sources was investigated. Fish were fed for 16 weeks with five experimental diets supplemented either with fish oil (FO), or one of four different vegetable oils: olive oil (OO), sunflower oil (SO), linseed oil (LO), and palm oil (PO). DNA adducts were measured by LC-ESI/MS/MS in the liver, spleen and kidney to evaluate DNA damage.The highest 8-oxodGuo levels were observed in liver and kidney of fish fed the FO diet (7814 ± 678 and 13,554 ± 1571 adducts/109 nucleosides, respectively), and in spleen of fish receiving the SO diet (13,541 adducts/109 nucleosides). The PO diet resulted in the lowest content of 8-oxodGuo in all tissues, 6720 ± 652 adducts/109 nucleosides in liver, 8340 ± 668 adducts/109 nucleosides in kidney, and 6202 ± 1038 adducts/109 nucleosides in spleen. Similarly, εdAdo was highest in the FO treatment, 12.5 ± 1.9 adducts/109 and 58.2 ± 4.6 adducts/109 nucleosides in liver and kidney respectively, whereas SO produced the highest εdAdo value (568.2 adducts/109 nucleosides) in spleen. In spleen and kidney, εdAdo was lowest in the PO treatment (47.8 and 27.1 ± 5.9 adducts/109, respectively). In liver, the OO diet registered the lowest εdAdo value (8.9 ± 1.9 adducts/109 nucleosides).Significant positive correlations were observed between levels of 8-oxodGuo in kidney tissues and the amount of 20:4n-6, 20:5n-3, 22:6n-3, n-3 LC-PUFA and n-6 LC-PUFA intake (P < 0.05). The εdAdo levels in spleen tissue were correlated with 18:2n-6 and n-6 PUFA intake (P < 0.05).This study suggests that dietary lipids play an important role in the oxidatively generated damage to DNA in fish.
