| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2423769 | Aquaculture | 2010 | 7 Pages |
We investigated genetic diversity and population structure of mrigal in Myanmar using microsatellite DNA markers. A total of 211 individuals from five wild populations and 216 individuals from five hatcheries were analysed for six microsatellite loci (Bgon22, Lr3, Lr12, Lr21, MFW1 and MFW17) which were developed for other cyprinids. For comparison, 43 individuals from a hatchery in northern Vietnam, of Indian origin and introduced in 1984, also were analysed. Tests for all loci revealed H–W equilibrium in only two hatchery samples. Allele richness ranged from 2.3 to 8.5. Overall, observed heterozygosity was high in all Myanmar samples (ranging from 0.654 to 0.756) but relatively low in the Vietnam hatchery sample (0.303). Pairwise FST values among the Myanmar samples ranged from 0.000 to 0.096, and those between the Myanmar and the Vietnam samples from 0.353 to 0.506. Results of multidimensional scaling analysis (MDS) of pairwise FST and Bayesian method revealed that one wild and two hatchery samples from Myanmar were differentiated from others, which appeared highly admixed. The study has important implications for genetic management of mrigal stocks in Myanmar, and possibly elsewhere in the region. For baseline stock for selective breeding, it would be best to include representation of samples from all groups we have identified to ensure a broad genetic base for genetic improvement programs. As for stock enhancement, seed produced from several hatcheries examined here should not be used for restocking in certain locations to avoid genetic contamination.
