| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2424401 | Aquaculture | 2009 | 4 Pages |
The quality of oyster spermatozoa has become an issue for modern aquaculture. However, reliable protocols used to assess gamete quality are lacking. The aim of this work is to define a standardised experimental fertilisation protocol for Pacific oyster oocytes.Six experiments have been carried out in this study. The optimal conditions for Pacific oyster experimental fertilisation have been defined: (1) oocytes can be conserved in seawater for at least 4 h before fertilisation, (2) oocyte concentration: 100–1000 ml− 1 seawater, (3) fertilisation volume: 10–100 ml, (4) spermatozoa:oocyte ratio: 400 to maintain discriminating conditions, (5) gamete contact time: longer than 10 min and (6) fertilisation and incubation temperatures: both 19 °C. The effect of individual male significantly influenced the fertilisation success. The results of this study will be exploited for spermatozoal quality analysis in combination with other techniques.
