| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2425290 | Aquaculture | 2007 | 6 Pages |
The cell wall hydrophobicity of Bacillus sp. YB-030518 and YB-034325 isolated from carp (Cyprinus carpio) ponds and its changes under different culture conditions were studied on the basis of the amount of bacteria in a hydrocarbon/water two-phase system. We investigated the effect of cell wall hydrophobicity on the role of these bacteria as probiotics in bioremediation based on shrimp feed. Culture conditions such as growth phase, pH and temperature influence the hydrophobic properties of the Bacillus sp. cell surface. In the exponential growth phase (18 h), the hydrophobicity of both YB-030518 and YB-034325 was significantly higher (P < 0.05) than that at 96 h. The hydrophobicity of YB-034325 was significantly higher (P < 0.05) than that of YB-030518 (5 °C at pH 5.5 and pH 8.5; 20 °C at pH 5.5 and pH 7.0). However, for any single probiotic, there was no statistical difference (P > 0.05) in cell wall hydrophobicity at different pH values (5.5, 7.0 and 8.5) or at different temperatures (5 °C and 20 °C). The relative growth rate of YB-034325 with a high level of hydrophobicity was significantly higher (P < 0.05) than that of YB-030518. The bioremediation capability at 48 h and 96 h was significantly higher in YB-034325 (P < 0.05) compared to YB-030518 and the Control. Moreover, the mean bioremediation capability of treatment with probiotics Bacillus sp. YB-030518 and YB-034325 was significantly higher (P < 0.05) than that of the Control.
