Development of a microsatellite multiplex PCR for Senegalese sole (Solea senegalensis) and its application to broodstock management

Pedigree data indicated that only one female and two males had contributed to the offspring, thus generating two half-sib families. Results showed how broodstock development using F1 descendants, in absence of genetic screening, might lead to a drastic reduction in the level of genetic variability and to a strong relatedness among breeders. It is known that these practices are generally applied on many farms, thus having putative negative consequences in the current poor performance shown by S. senegalensis.