Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide

We determined whether a major Japanese cedar pollen allergen (Cry j 1) conjugated with CpG oligodeoxynucleotide would enhance allergen-specific Th1 responses in mice. Cry j 1 conjugated with CpG (Cry j 1–CpG) induced IL-12 in the spleen cells of naïve mice. Cry j 1–CpG immunization of BALB/c mice suppressed anti-Cry j 1 IgE response and enhanced anti-Cry j 1 IgG2a to subsequent Cry j 1 and alum adjuvant injection. CD4+T cells isolated from the spleens in mice immunized with Cry j 1–CpG produced higher IFN-γ levels than did CD4+T cells obtained from mice as negative controls. Our results suggested that Cry j 1–CpG immunization can induce Cry j 1-specific Th1 immune responses, thereby inhibiting IgE response to the pollen allergen.