Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2439272 | Journal of Dairy Science | 2009 | 5 Pages |
Abstract
Three adult lactating Holstein cows were injected in the subcutaneous abdominal vein with 175Â ng/kg of body weight of Clostridium botulinum type C toxin (451 cow median toxic doses) to determine if this botulinum toxin crosses the blood-milk barrier. Whole blood (in sodium heparin) and clotted blood serum samples were taken at 0Â min, 10Â min, and 3, 6, 9, and 12Â h postinoculation. Milk samples were taken at 0Â min and at 3, 6, 9 and 12Â h postinoculation. All samples were tested for the presence of the toxin using the mouse bioassay and immunostick ELISA test. The immunostick ELISA identified the toxin in whole blood and the mouse bioassay identified the toxin in serum at all times examined in all 3 animals. Toxin was not identified by either detection method in milk samples collected from the 3 animals. From these results, it appears that Clostridium botulinum type C toxin does not cross from the blood to the milk in detectable concentrations.
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Authors
R.B. Jr., B. Puschner, R.L. Walker, T.E. Rocke, S.R. Smith, J.S. Cullor, A.A. Ardans,